Description
Intended Uses: This kit is for the qualitative detection of Human Anti-Herpes Simplex Virus 1 IgG (also known as HSV-1 IgG) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principle of the Assay: The microtiter plate has been pre-coated with a target antigen. Positive/Negative Controls or samples are added to the wells and incubate. Antibodies in the samples bind to the antigen. Unbound antibody is washed away during a washing step. Avidin-Horseradish Peroxidase (HRP) conjugated is then added and incubate. Unbound avidin-HRP is washed away during a washing step. Substrate Solution is then added and color develops. The reaction is stopped by addition of acidic stop solution and color changes into yellow that can be measured at 450 nm